Hotspot

trnK-AAA-rps16

LSC · 4272-4919 · 648 bp

Back to Murbeckiella

Pi 0.0141

MarkerSeek score 68.30

Primer available yes

Label trnK-AAA-rps16

Feature Metadata

label_name: trnK-AAA-rps16
feature_type: igs
region: LSC
length_bp: 648
variable_sites: 9
pi: 0.0141
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 68.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-AAA-rps16_p1	AACTCTACCGCAGATCTGGA	AAGGCAGGGATTTTTACGGA	50.0	45.0	57.9	57.1	791-801 bp	1.0	73.6
trnK-AAA-rps16_p2	AACTCTACCGCAGATCTGGA	AGGCAGGGATTTTTACGGAA	50.0	45.0	57.9	57.1	790-800 bp	1.0	73.6
trnK-AAA-rps16_p3	AAAGCCGAGTACTCTACCGT	AGGCAGGGATTTTTACGGAA	50.0	45.0	58.2	57.1	722-732 bp	1.0	73.6
trnK-AAA-rps16_p4	AAGCCGAGTACTCTACCGTT	AAGGCAGGGATTTTTACGGA	50.0	45.0	58.2	57.1	722-732 bp	1.0	73.6
trnK-AAA-rps16_p5	AAGCCGAGTACTCTACCGTT	AGGCAGGGATTTTTACGGAA	50.0	45.0	58.2	57.1	721-731 bp	1.0	73.6