Hotspot

ycf1

SSC · 126912-132536 · 5625 bp

Back to Mukdenia

Pi 0.00214

MarkerSeek score 53.90

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5625
variable_sites: 12
pi: 0.00214
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	AAGTTCCCATGGAATTCCCG	ACGAAAACAACCTAAGCAACG	50.0	42.9	57.9	57.7	234 bp	1.0	68.9
ycf1_p2	TTCCCATGGAATTCCCGATG	ACGAAAACAACCTAAGCAACG	50.0	42.9	57.6	57.7	231 bp	1.0	67.2
ycf1_p3	GTTCCCATGGAATTCCCGAT	ACGAAAACAACCTAAGCAACG	50.0	42.9	57.6	57.7	232 bp	1.0	67.1
ycf1_p4	AAGTTCCCATGGAATTCCCG	CGAAAACAACCTAAGCAACGA	50.0	42.9	57.9	57.4	233 bp	1.0	66.8
ycf1_p5	AGCAAGAAGGATACCGTGGA	ACGAAAACAACCTAAGCAACG	50.0	42.9	58.4	57.7	269 bp	1.0	66.5