Hotspot

atpF

LSC · 12585-13883 · 1299 bp

Back to Mukdenia

Pi 0.00311

MarkerSeek score 61.50

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1299
variable_sites: 4
pi: 0.00311
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	GCTCGAATGGTTACCATGAGT	GGGGGCGAAGTGATACAAAA	47.6	50.0	58.1	58.2	1432-1445 bp	1.0	70.8
atpF_p2	CAGCTCGAATGGTTACCATGA	GGGGGCGAAGTGATACAAAA	47.6	50.0	58.1	58.2	1434-1447 bp	1.0	70.8
atpF_p3	CATCAGCTCGAATGGTTACCA	GGGGGCGAAGTGATACAAAA	47.6	50.0	58.1	58.2	1437-1450 bp	1.0	70.8
atpF_p4	TCAGCTCGAATGGTTACCATG	GGGGGCGAAGTGATACAAAA	47.6	50.0	58.1	58.2	1435-1448 bp	1.0	70.8
atpF_p5	GCTCGATACGTTCCCGGATA	GGGGGCGAAGTGATACAAAA	55.0	50.0	59.1	58.2	1470-1483 bp	1.0	70.6