Hotspot

trnM-CAU-atpE

LSC · 55304-55472 · 169 bp

Back to Mukdenia

Pi 0.0536

MarkerSeek score 77.00

Primer available yes

Label trnM-CAU-atpE

Feature Metadata

label_name: trnM-CAU-atpE
feature_type: igs
region: LSC
length_bp: 169
variable_sites: 9
pi: 0.0536
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 77.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnM-CAU-atpE_p1	GGCAGGAGTCATTGGTTCAA	CTCTCAGACGAGCTAGGACA	50.0	55.0	58.1	58.0	275-338 bp	1.0	81.6
trnM-CAU-atpE_p2	GGCAGGAGTCATTGGTTCAA	CGAGCTAGGACACGAGTAGA	50.0	55.0	58.1	58.1	267-330 bp	1.0	81.5
trnM-CAU-atpE_p3	GGCAGGAGTCATTGGTTCAA	GAACTAATTGGTGTGCCCGA	50.0	50.0	58.1	58.2	228-291 bp	1.0	80.9
trnM-CAU-atpE_p4	GGCAGGAGTCATTGGTTCAA	TCGGAACTAATTGGTGTGCC	50.0	50.0	58.1	58.2	231-294 bp	1.0	80.9
trnM-CAU-atpE_p5	TTGCTTTCATACGGCAGGAG	CTCTCAGACGAGCTAGGACA	50.0	55.0	58.3	58.0	287-350 bp	1.0	80.2