Hotspot

psbC-trnS-UGA

LSC · 37290-37535 · 246 bp

Back to Mukdenia

Pi 0.0285

MarkerSeek score 61.40

Primer available yes

Label psbC-trnS-UGA

Feature Metadata

label_name: psbC-trnS-UGA
feature_type: igs
region: LSC
length_bp: 246
variable_sites: 7
pi: 0.0285
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC-trnS-UGA_p1	CCTGTTCTTTCCATGACCCC	GAGAGATGGCTGAGTGGTTG	55.0	55.0	58.2	58.0	368 bp	1.0	80.1
psbC-trnS-UGA_p2	CCTGTTCTTTCCATGACCCC	GTCTTTCGGAGAGATGGCTG	55.0	55.0	58.2	58.1	376 bp	1.0	79.7
psbC-trnS-UGA_p3	TCGTAGGTCATTTGTGGCAC	GAGAGATGGCTGAGTGGTTG	50.0	55.0	58.2	58.0	448 bp	1.0	79.5
psbC-trnS-UGA_p4	CCTGTTCTTTCCATGACCCC	ATAGCCCCGGTCTTGAAAAC	55.0	50.0	58.2	57.9	348 bp	1.0	79.3
psbC-trnS-UGA_p5	TCGTAGGTCATTTGTGGCAC	GTCTTTCGGAGAGATGGCTG	50.0	55.0	58.2	58.1	456 bp	1.0	79.1