Hotspot

trnC-GCA-petN

LSC · 29039-29896 · 858 bp

Back to Mukdenia

Pi 0.00716

MarkerSeek score 65.10

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 858
variable_sites: 6
pi: 0.00716
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 65.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTCCCCAGTTCAAATCCGG	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.1	58.2	914-919 bp	1.0	80.4
trnC-GCA-petN_p2	TTTCCCCAGTTCAAATCCGG	AGAGTCCACTTCTTCCCCAT	50.0	50.0	58.1	57.6	975-980 bp	1.0	78.7
trnC-GCA-petN_p3	GGGGGACTGCAAATCCTTTT	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.4	58.2	932-937 bp	1.0	78.2
trnC-GCA-petN_p4	ATTTTGGATCGTTTTGGCGG	AGCCCAAGCGAGACTTACTA	45.0	50.0	57.6	58.2	971-976 bp	1.0	78.0
trnC-GCA-petN_p5	GGGGGACTGCAAATCCTTTT	AGAGTCCACTTCTTCCCCAT	50.0	50.0	58.4	57.6	993-998 bp	1.0	76.4