Hotspot

rps16-trnQ-UUG

LSC · 6051-7738 · 1688 bp

Back to Mukdenia

Pi 0.00435

MarkerSeek score 65.30

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1688
variable_sites: 7
pi: 0.00435
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 65.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AAGTCGCACGTTGCTTTCTA	GAAATCAAAATGGGGCGTGG	45.0	50.0	58.1	58.0	1746-1819 bp	1.0	82.6
rps16-trnQ-UUG_p2	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	1688-1761 bp	1.0	82.2
rps16-trnQ-UUG_p3	TCATGTCCTTCAAGTCGCAC	GAAATCAAAATGGGGCGTGG	50.0	50.0	58.2	58.0	1757-1830 bp	1.0	82.0
rps16-trnQ-UUG_p4	TCATGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	1699-1772 bp	1.0	81.6
rps16-trnQ-UUG_p5	CCACAGCTGATCATGTCCTT	GAAATCAAAATGGGGCGTGG	50.0	50.0	57.6	58.0	1767-1840 bp	1.0	80.3