Hotspot

ycf1

SSC · 124553-130036 · 5484 bp

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Pi 0.0234

MarkerSeek score 52.80

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5484
variable_sites: 128
pi: 0.0234
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TCTCTACGGCGTTTAGGGAA	CCCCGTCGTTTGTCTGTAAA	50.0	50.0	58.2	58.1	239 bp	1.0	81.0
ycf1_p2	TTCTCTACGGCGTTTAGGGA	CCCCGTCGTTTGTCTGTAAA	50.0	50.0	58.2	58.1	240 bp	1.0	81.0
ycf1_p3	TCTCTACGGCGTTTAGGGAA	ACAGAATTGACGAAAGGGGG	50.0	50.0	58.2	57.8	275 bp	1.0	80.0
ycf1_p4	TTCTCTACGGCGTTTAGGGA	ACAGAATTGACGAAAGGGGG	50.0	50.0	58.2	57.8	276 bp	1.0	80.0
ycf1_p5	TCTCTACGGCGTTTAGGGAA	GACGAAAGGGGGAATGCTTA	50.0	50.0	58.2	57.6	267 bp	1.0	78.4