Hotspot

ndhD-psaC

SSC · 117698-117892 · 195 bp

Back to Muiriantha

Pi 0.0426

MarkerSeek score 67.20

Primer available yes

Label ndhD-psaC

Feature Metadata

label_name: ndhD-psaC
feature_type: igs
region: SSC
length_bp: 195
variable_sites: 8
pi: 0.0426
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 67.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-psaC_p1	CGCGGCTATCGGAAAAACTA	ACTAGAAGCATGGGTCTAGCT	50.0	47.6	58.4	58.0	240-246 bp	1.0	73.1
ndhD-psaC_p2	CGCGGCTATCGGAAAAACTA	ACTAGAAGCATGGGTCTAGC	50.0	50.0	58.4	56.4	240-246 bp	1.0	68.8
ndhD-psaC_p3	GCGGCTATCGGAAAAACTAC	ACTAGAAGCATGGGTCTAGCT	50.0	47.6	57.0	58.0	239-245 bp	1.0	68.5
ndhD-psaC_p4	CGCGGCTATCGGAAAAACTA	AACTAGAAGCATGGGTCTAGC	50.0	47.6	58.4	57.2	241-247 bp	1.0	66.8
ndhD-psaC_p5	CGCGGCTATCGGAAAAACTA	ACAACTAGAAGCATGGGTCT	50.0	45.0	58.4	56.1	243-249 bp	1.0	66.6