Hotspot

rpl32-trnL-UAG

SSC · 114158-114788 · 631 bp

Back to Muiriantha

Pi 0.0265

MarkerSeek score 64.90

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 631
variable_sites: 16
pi: 0.0265
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 64.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GCGTTGAAGGCTTTTTCGTT	AGCGTGTCTACCGATTTCAC	45.0	50.0	58.2	58.0	728-753 bp	1.0	83.7
rpl32-trnL-UAG_p2	GCGTTGAAGGCTTTTTCGTT	CAGCGTGTCTACCGATTTCA	45.0	50.0	58.2	58.0	729-754 bp	1.0	83.7
rpl32-trnL-UAG_p3	GCGTTGAAGGCTTTTTCGTT	TGCCTCGAGTAAATCCAACG	45.0	50.0	58.2	58.0	1071-1097 bp	1.0	82.3
rpl32-trnL-UAG_p4	GCGTTGAAGGCTTTTTCGTT	CTTGGTGCCGTTATAGTGCT	45.0	50.0	58.2	58.0	1191-1217 bp	1.0	81.9
rpl32-trnL-UAG_p5	GCGTTGAAGGCTTTTTCGTT	CCTCGAGTAAATCCAACGGG	45.0	55.0	58.2	58.1	1069-1095 bp	1.0	81.8