Hotspot

rpl14-rpl16

LSC · 81703-81824 · 122 bp

Back to Muiriantha

Pi 0.041

MarkerSeek score 67.10

Primer available yes

Label rpl14-rpl16

Feature Metadata

label_name: rpl14-rpl16
feature_type: igs
region: LSC
length_bp: 122
variable_sites: 5
pi: 0.041
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl14-rpl16_p1	CCGCTATTGTCCGCTACATT	CATCGCTCGAAGGGCTATTT	50.0	50.0	58.1	58.1	239-249 bp	1.0	79.5
rpl14-rpl16_p2	CCGCTATTGTCCGCTACATT	ATCGCTCGAAGGGCTATTTC	50.0	50.0	58.1	57.8	238-248 bp	1.0	79.3
rpl14-rpl16_p3	CCGCTATTGTCCGCTACATT	CAATAGCGGCGTCCAAAATG	50.0	50.0	58.1	58.2	219-229 bp	1.0	79.3
rpl14-rpl16_p4	CCGCTATTGTCCGCTACATT	TCAATAGCGGCGTCCAAAAT	50.0	45.0	58.1	58.3	220-230 bp	1.0	78.7
rpl14-rpl16_p5	CCGCTATTGTCCGCTACATT	ATTTCAATAGCGGCGTCCAA	50.0	45.0	58.1	58.3	223-233 bp	1.0	78.6