Hotspot

rps11-rpl36

LSC · 80222-80351 · 130 bp

Back to Muiriantha

Pi 0.0469

MarkerSeek score 73.10

Primer available yes

Label rps11-rpl36

Feature Metadata

label_name: rps11-rpl36
feature_type: igs
region: LSC
length_bp: 130
variable_sites: 6
pi: 0.0469
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 73.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps11-rpl36_p1	AACCAATCCGTCCAGTCCTA	AAAATGTCGACTGATCCGCA	50.0	45.0	58.1	57.9	259-261 bp	1.0	81.3
rps11-rpl36_p2	CGACGTCTACTTTTACGCGA	AAAATGTCGACTGATCCGCA	50.0	45.0	58.1	57.9	278-280 bp	1.0	80.5
rps11-rpl36_p3	TCGACGTCTACTTTTACGCG	AAAATGTCGACTGATCCGCA	50.0	45.0	58.1	57.9	279-281 bp	1.0	80.5
rps11-rpl36_p4	CTTTTACGCGAACCAATCCG	AAAATGTCGACTGATCCGCA	50.0	45.0	57.8	57.9	269-271 bp	1.0	80.4
rps11-rpl36_p5	AACCAATCCGTCCAGTCCTA	GCAGGAGGGGACGGATTATA	50.0	55.0	58.1	58.4	242-244 bp	1.0	79.3