Hotspot

trnfM-CAU-rps14

LSC · 37751-37913 · 163 bp

Back to Muiriantha

Pi 0.0491

MarkerSeek score 67.90

Primer available yes

Label trnfM-CAU-rps14

Feature Metadata

label_name: trnfM-CAU-rps14
feature_type: igs
region: LSC
length_bp: 163
variable_sites: 8
pi: 0.0491
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnfM-CAU-rps14_p1	GCTACCAAACTGCTCTACCC	GGGGGCAACAAGATCAAGTT	55.0	50.0	58.0	58.4	212-214 bp	1.0	79.9
trnfM-CAU-rps14_p2	AAGGTTATGAGCCTTGCGAG	GGGGGCAACAAGATCAAGTT	50.0	50.0	58.0	58.4	231-233 bp	1.0	79.6
trnfM-CAU-rps14_p3	TTTGAACCCGTGACCTCAAG	GGGGGCAACAAGATCAAGTT	50.0	50.0	58.0	58.4	248-250 bp	1.0	79.3
trnfM-CAU-rps14_p4	AAAATGTTGCTGTTGCGGAG	GGGGGCAACAAGATCAAGTT	45.0	50.0	57.9	58.4	274-276 bp	1.0	78.3
trnfM-CAU-rps14_p5	GATTTGAACCCGTGACCTCA	GGGGGCAACAAGATCAAGTT	50.0	50.0	57.8	58.4	250-252 bp	1.0	78.2