Hotspot

psbZ-trnG-GCC

LSC · 36923-37442 · 520 bp

Back to Muiriantha

Pi 0.0308

MarkerSeek score 68.70

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 520
variable_sites: 16
pi: 0.0308
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	CGGATGGTTGGTCGAGTAAC	AATCGAACCCGCATCTTCTC	55.0	50.0	58.4	58.1	678-679 bp	1.0	78.6
psbZ-trnG-GCC_p2	CGGATGGTTGGTCGAGTAAC	GAATCGAACCCGCATCTTCT	55.0	50.0	58.4	58.1	679-680 bp	1.0	78.6
psbZ-trnG-GCC_p3	CGGATGGTTGGTCGAGTAAC	AGTCCTTTACTCTGGCGGAT	55.0	50.0	58.4	58.1	704-705 bp	1.0	78.0
psbZ-trnG-GCC_p4	TTTGCTTCTCCGGATGGTTG	AATCGAACCCGCATCTTCTC	50.0	50.0	58.5	58.1	688-689 bp	1.0	77.8
psbZ-trnG-GCC_p5	TTTGCTTCTCCGGATGGTTG	GAATCGAACCCGCATCTTCT	50.0	50.0	58.5	58.1	689-690 bp	1.0	77.8