Hotspot

trnR-UCU-atpA

LSC · 9483-9675 · 193 bp

Back to Muiriantha

Pi 0.0466

MarkerSeek score 69.90

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 193
variable_sites: 9
pi: 0.0466
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	CTGAAAGGCGTCCATTGTCT	GAGGAAGCAGAAGCCCTTTT	50.0	50.0	58.2	58.1	347 bp	1.0	79.9
trnR-UCU-atpA_p2	CTGAAAGGCGTCCATTGTCT	GAAGCCCTTTTGCAAGAAGC	50.0	50.0	58.2	58.2	338 bp	1.0	78.9
trnR-UCU-atpA_p3	CTGAAAGGCGTCCATTGTCT	AGCCCTTTTGCAAGAAGCTA	50.0	45.0	58.2	57.7	336 bp	1.0	78.3
trnR-UCU-atpA_p4	ACTGAAAGGCGTCCATTGTC	GAGGAAGCAGAAGCCCTTTT	50.0	50.0	58.5	58.1	348 bp	1.0	77.6
trnR-UCU-atpA_p5	GACTGAAAGGCGTCCATTGT	GAGGAAGCAGAAGCCCTTTT	50.0	50.0	58.5	58.1	349 bp	1.0	77.6