Hotspot

rps16

LSC · 4497-5602 · 1106 bp

Back to Muhlenbergia

Pi 0.00181

MarkerSeek score 51.40

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1106
variable_sites: 3
pi: 0.00181
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	AAGGGATATTAACGACCCCC	TCTTGATACCACTTTGCAATGA	50.0	36.4	56.4	56.0	1199-1200 bp	1.0	40.2
rps16_p2	AAGGGATATTAACGACCCCC	ACCACTTTGCAATGAAATTAGAGG	50.0	37.5	56.4	58.0	1192-1193 bp	1.0	40.2
rps16_p3	AAGGGATATTAACGACCCCC	TCCACATTTCTCCATAGATAGCA	50.0	39.1	56.4	56.8	1281-1282 bp	1.0	40.2
rps16_p4	AAGGGATATTAACGACCCCC	TGCAATGAAATTAGAGGGATGT	50.0	36.4	56.4	55.8	1185-1186 bp	1.0	40.2
rps16_p5	AAGGGATATTAACGACCCCC	CACTTTGCAATGAAATTAGAGGGA	50.0	37.5	56.4	57.7	1190-1191 bp	1.0	40.2