Hotspot

rpoC2

LSC · 25030-29550 · 4521 bp

Back to Muhlenbergia

Pi 0.000737

MarkerSeek score 41.20

Primer available yes

Label rpoC2

Feature Metadata

label_name: rpoC2
feature_type: gene
region: LSC
length_bp: 4521
variable_sites: 5
pi: 0.000737
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 41.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2_p1	TTCGTTCGAACTTGCTTGGT	TCAACAAGGTGCCAATAGCT	45.0	45.0	58.3	57.4	277 bp	1.0	73.2
rpoC2_p2	TCGTTCGAACTTGCTTGGTA	TCAACAAGGTGCCAATAGCT	45.0	45.0	57.5	57.4	276 bp	1.0	71.8
rpoC2_p3	GAAGAAAAAGAGGGGGCTCG	TCAACAAGGTGCCAATAGCT	55.0	45.0	58.5	57.4	241 bp	1.0	71.6
rpoC2_p4	TTCGTTCGAACTTGCTTGGT	ACAAGGTGCCAATAGCTTCT	45.0	45.0	58.3	57.1	274 bp	1.0	70.8
rpoC2_p5	TCGTTCGAACTTGCTTGGTA	ACAAGGTGCCAATAGCTTCT	45.0	45.0	57.5	57.1	273 bp	1.0	69.4