Hotspot

ccsA

SSC · 104695-105663 · 969 bp

Back to Muhlenbergia

Pi 0.00138

MarkerSeek score 33.80

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 969
variable_sites: 2
pi: 0.00138
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 33.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	ACCAAAAGGTTTTTCGGGGT	TCTGGACCACGAGAACTCTT	45.0	50.0	58.1	58.0	2002-2015 bp	1.0	79.6
ccsA_p2	ACCAAAAGGTTTTTCGGGGT	TTCTGGACCACGAGAACTCT	45.0	50.0	58.1	58.0	2003-2016 bp	1.0	79.6
ccsA_p3	TAGACACGCTGCTCTTAGGA	TCTGGACCACGAGAACTCTT	50.0	50.0	57.9	58.0	1434 bp	1.0	79.3
ccsA_p4	TAGACACGCTGCTCTTAGGA	TTCTGGACCACGAGAACTCT	50.0	50.0	57.9	58.0	1435 bp	1.0	79.3
ccsA_p5	TAGACACGCTGCTCTTAGGA	GGATTCTGGACCACGAGAAC	50.0	55.0	57.9	58.0	1438 bp	1.0	79.2