Hotspot

rps11-rpl36

LSC · 75222-75396 · 175 bp

Back to Muhlenbergia

Pi 0

MarkerSeek score 52.50

Primer available yes

Label rps11-rpl36

Feature Metadata

label_name: rps11-rpl36
feature_type: igs
region: LSC
length_bp: 175
variable_sites: 0
pi: 0
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 52.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps11-rpl36_p1	CCGTGCACTTTTCCGTAAAC	GCGTGGTCGAATTAGAGTCA	50.0	50.0	58.0	57.7	324-326 bp	1.0	78.1
rps11-rpl36_p2	CGCAAACCAATACGCACTTT	GCGTGGTCGAATTAGAGTCA	45.0	50.0	58.0	57.7	287-289 bp	1.0	78.0
rps11-rpl36_p3	TCCGTGCACTTTTCCGTAAA	GCGTGGTCGAATTAGAGTCA	45.0	50.0	58.0	57.7	325-327 bp	1.0	77.9
rps11-rpl36_p4	CTACGCAAACCAATACGCAC	GCGTGGTCGAATTAGAGTCA	50.0	50.0	57.8	57.7	290-292 bp	1.0	77.1
rps11-rpl36_p5	CCGTGCACTTTTCCGTAAAC	GGCGTGGTCGAATTAGAGTC	50.0	55.0	58.0	58.4	325-327 bp	1.0	76.7