Hotspot

petA-psbJ

LSC · 59870-60805 · 936 bp

Back to Muhlenbergia

Pi 0.00642

MarkerSeek score 48.80

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 936
variable_sites: 9
pi: 0.00642
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GCAATCCTAATGTGGGAGGG	TGCGGGTTGGTTATTTGTCA	55.0	45.0	58.0	58.0	1783-1800 bp	1.0	80.3
petA-psbJ_p2	GCGGAAATAGTGCTTCAGGA	TGCGGGTTGGTTATTTGTCA	50.0	45.0	58.0	58.0	1748-1765 bp	1.0	80.2
petA-psbJ_p3	CTTGTGATAGGCCCTGTTCC	TGCGGGTTGGTTATTTGTCA	55.0	45.0	58.0	58.0	2132-2149 bp	1.0	80.0
petA-psbJ_p4	GCAATCCTAATGTGGGAGGG	GTCCTCGGCCAAACGAATAT	55.0	50.0	58.0	58.1	1733-1750 bp	1.0	80.0
petA-psbJ_p5	TGCTTCAGGATCCATTACGC	TGCGGGTTGGTTATTTGTCA	50.0	45.0	58.0	58.0	1738-1755 bp	1.0	80.0