Hotspot

ndhC-trnV-UAC

LSC · 49688-50600 · 913 bp

Back to Muhlenbergia

Pi 0.00296
MarkerSeek score 62.10
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
913
variable_sites
4
pi
0.00296
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TCCTTCACTAATCGGGGCTA GGTTCGAATCCGTATAGCCC 50.0 55.0 57.5 57.9 1032-1046 bp 1.0 77.1
ndhC-trnV-UAC_p2 TCCTTCACTAATCGGGGCTA AGCCCAATGCAGAAAAATGC 50.0 45.0 57.5 57.9 1125-1139 bp 1.0 76.7
ndhC-trnV-UAC_p3 CTGGTCCTTCACTAATCGGG GGTTCGAATCCGTATAGCCC 55.0 55.0 57.4 57.9 1036-1050 bp 1.0 75.9
ndhC-trnV-UAC_p4 CTGGTCCTTCACTAATCGGG AGCCCAATGCAGAAAAATGC 55.0 45.0 57.4 57.9 1129-1143 bp 1.0 75.6
ndhC-trnV-UAC_p5 TCCTTCACTAATCGGGGCTA CGGTTCGAATCCGTATAGCC 50.0 55.0 57.5 58.3 1033-1047 bp 1.0 75.2