Hotspot

ndhJ-ndhK

LSC · 48474-48587 · 114 bp

Back to Muhlenbergia

Pi 0.00601

MarkerSeek score 59.70

Primer available yes

Label ndhJ-ndhK

Feature Metadata

label_name: ndhJ-ndhK
feature_type: igs
region: LSC
length_bp: 114
variable_sites: 1
pi: 0.00601
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhJ-ndhK_p1	GGTGATCGAAGCCCAAAGAT	CCGGAACTTACGAGCAAGAA	50.0	50.0	58.0	57.9	296-300 bp	1.0	80.4
ndhJ-ndhK_p2	GATAACGATCCCTGCTGCAT	CCGGAACTTACGAGCAAGAA	50.0	50.0	57.8	57.9	243-247 bp	1.0	79.6
ndhJ-ndhK_p3	GAATCCCAATCCCCTGCTTT	CCGGAACTTACGAGCAAGAA	50.0	50.0	57.8	57.9	339-343 bp	1.0	79.3
ndhJ-ndhK_p4	TAGAATCCCAATCCCCTGCT	CCGGAACTTACGAGCAAGAA	50.0	50.0	57.8	57.9	341-345 bp	1.0	78.9
ndhJ-ndhK_p5	GGTGATCGAAGCCCAAAGAT	CATACCGGAACTTACGAGCA	50.0	50.0	58.0	57.4	300-304 bp	1.0	76.4