Hotspot

trnS-GGA-rps4

LSC · 44808-45068 · 261 bp

Back to Muhlenbergia

Pi 0.0026

MarkerSeek score 56.30

Primer available yes

Label trnS-GGA-rps4

Feature Metadata

label_name: trnS-GGA-rps4
feature_type: igs
region: LSC
length_bp: 261
variable_sites: 1
pi: 0.0026
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 56.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GGA-rps4_p1	ATGTAGGAGAGATGGCCGAG	GAAAGTGGGTCGGTCTGAAA	55.0	50.0	58.4	57.8	412-418 bp	1.0	76.4
trnS-GGA-rps4_p2	ATGTAGGAGAGATGGCCGAG	TAGAAAGTGGGTCGGTCTGA	55.0	50.0	58.4	57.7	414-420 bp	1.0	76.0
trnS-GGA-rps4_p3	ATGTAGGAGAGATGGCCGAG	AGAAAGTGGGTCGGTCTGAA	55.0	50.0	58.4	58.6	413-419 bp	1.0	73.7
trnS-GGA-rps4_p4	ATGTAGGAGAGATGGCCGAG	AAAGTGGGTCGGTCTGAAAA	55.0	45.0	58.4	57.3	411-417 bp	1.0	72.6
trnS-GGA-rps4_p5	TTCGAATCCCTCTCTTTCCG	GAAAGTGGGTCGGTCTGAAA	50.0	50.0	57.0	57.8	340-346 bp	1.0	71.9