Hotspot

trnG-UCC-trnT-GGU

LSC · 13490-14475 · 986 bp

Back to Muhlenbergia

Pi 0.00205

MarkerSeek score 37.60

Primer available yes

Label trnG-UCC-trnT-GGU

Feature Metadata

label_name: trnG-UCC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 986
variable_sites: 3
pi: 0.00205
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 37.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-trnT-GGU_p1	ACGAATCACACTTTTACCACT	TTGATCTTGCCGCGACATTA	38.1	45.0	55.5	58.0	1121-1130 bp	1.0	52.4
trnG-UCC-trnT-GGU_p2	ACGAATCACACTTTTACCACT	TTGCCGCGACATTACTCTAC	38.1	50.0	55.5	58.1	1115-1124 bp	1.0	51.9
trnG-UCC-trnT-GGU_p3	AGAACGAATCACACTTTTACCAC	TTGATCTTGCCGCGACATTA	39.1	45.0	57.3	58.0	1124-1133 bp	1.0	51.4
trnG-UCC-trnT-GGU_p4	GAACGAATCACACTTTTACCACT	TTGATCTTGCCGCGACATTA	39.1	45.0	57.3	58.0	1123-1132 bp	1.0	51.4
trnG-UCC-trnT-GGU_p5	ACGAATCACACTTTTACCACT	CCGCGACATTACTCTACCAC	38.1	55.0	55.5	58.2	1112-1121 bp	1.0	51.3