Hotspot

trnG-GCC-trnfM-CAU

LSC · 12197-12559 · 363 bp

Back to Muhlenbergia

Pi 0.00367

MarkerSeek score 53.80

Primer available yes

Label trnG-GCC-trnfM-CAU

Feature Metadata

label_name: trnG-GCC-trnfM-CAU
feature_type: igs
region: LSC
length_bp: 363
variable_sites: 2
pi: 0.00367
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-GCC-trnfM-CAU_p1	GAAGGTACGGGTTCGATTCC	ACCTGTTTTTGGCCCTACAG	55.0	50.0	58.1	58.0	488-491 bp	1.0	79.7
trnG-GCC-trnfM-CAU_p2	GCGAGCGTAGTTCAATGGTA	ACCTGTTTTTGGCCCTACAG	50.0	50.0	58.1	58.0	528-531 bp	1.0	79.6
trnG-GCC-trnfM-CAU_p3	AATATATGCCGTAGCGAGCG	ACCTGTTTTTGGCCCTACAG	50.0	50.0	58.1	58.0	541-544 bp	1.0	79.5
trnG-GCC-trnfM-CAU_p4	GACCCAGGCGGATATATCCT	ACCTGTTTTTGGCCCTACAG	55.0	50.0	58.1	58.0	572-575 bp	1.0	79.2
trnG-GCC-trnfM-CAU_p5	CGTAGCGAGCGTAGTTCAAT	ACCTGTTTTTGGCCCTACAG	50.0	50.0	58.2	58.0	532-535 bp	1.0	78.9