Hotspot

psbA

LSC · 232-1293 · 1062 bp

Back to Muellerina

Pi 0.00188

MarkerSeek score 48.20

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 2
pi: 0.00188
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	GCTCCTTTACTTTCAAGAACTCA	TCATGCTTGGGAGTCTCTGA	39.1	50.0	56.7	58.1	1149 bp	0.5	25.4
psbA_p2	GCTCCTTTACTTTCAAGAACTCA	TGCTTGGGAGTCTCTGATGA	39.1	50.0	56.7	58.1	1146 bp	0.5	25.4
psbA_p3	TGCTCCTTTACTTTCAAGAACTC	TCATGCTTGGGAGTCTCTGA	39.1	50.0	56.7	58.1	1150 bp	0.5	25.4
psbA_p4	TGCTCCTTTACTTTCAAGAACTC	TGCTTGGGAGTCTCTGATGA	39.1	50.0	56.7	58.1	1147 bp	0.5	25.4
psbA_p5	TGCTCCTTTACTTTCAAGAACTCA	TGCTTGGGAGTCTCTGATGA	37.5	50.0	58.3	58.1	1147 bp	0.5	25.0