Hotspot

trnL-UUG-ycf15

IRa · 117096-117639 · 544 bp

Back to Muellerina

Pi 0.0149

MarkerSeek score 67.60

Primer available yes

Label trnL-UUG-ycf15

Feature Metadata

label_name: trnL-UUG-ycf15
feature_type: igs
region: IRa
length_bp: 544
variable_sites: 8
pi: 0.0149
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 67.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UUG-ycf15_p1	AAATGGTAGACACGCGAGAC	ACGCATGTCTGATGAAATGGT	50.0	42.9	58.0	58.3	675-683 bp	1.0	71.5
trnL-UUG-ycf15_p2	TGAAATGGTAGACACGCGAG	ACGCATGTCTGATGAAATGGT	50.0	42.9	58.0	58.3	677-685 bp	1.0	71.5
trnL-UUG-ycf15_p3	GCCTTGGTGGTGAAATGGTA	ACGCATGTCTGATGAAATGGT	50.0	42.9	58.1	58.3	687-695 bp	1.0	70.9
trnL-UUG-ycf15_p4	TGGTGAAATGGTAGACACGC	ACGCATGTCTGATGAAATGGT	50.0	42.9	58.2	58.3	680-688 bp	1.0	70.0
trnL-UUG-ycf15_p5	AAATGGTAGACACGCGAGAC	CGCATGTCTGATGAAATGGT	50.0	45.0	58.0	56.5	674-682 bp	1.0	69.8