Hotspot

psaC-ycf1

SSC · 98410-99497 · 1088 bp

Back to Muellerina

Pi 0.0234

MarkerSeek score 63.90

Primer available yes

Label psaC-ycf1

Feature Metadata

label_name: psaC-ycf1
feature_type: igs
region: SSC
length_bp: 1088
variable_sites: 25
pi: 0.0234
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 63.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ycf1_p1	CACATTGAGTGCACCCGATA	TGGGTTAGTCCAATCAAATTGC	50.0	40.9	58.0	57.2	1162-1182 bp	1.0	60.5
psaC-ycf1_p2	CATTGAGTGCACCCGATACA	TGGGTTAGTCCAATCAAATTGC	50.0	40.9	58.0	57.2	1160-1180 bp	1.0	60.5
psaC-ycf1_p3	TTGAGTGCACCCGATACATG	TGGGTTAGTCCAATCAAATTGC	50.0	40.9	58.0	57.2	1158-1178 bp	1.0	60.5
psaC-ycf1_p4	ACATTGAGTGCACCCGATAC	TGGGTTAGTCCAATCAAATTGC	50.0	40.9	58.0	57.2	1161-1181 bp	1.0	60.5
psaC-ycf1_p5	TTGAGTGCACCCGATACATG	GGGTTAGTCCAATCAAATTGCT	50.0	40.9	58.0	56.9	1157-1177 bp	1.0	58.3