Hotspot

rbcL-accD

LSC · 47688-48153 · 466 bp

Back to Muellerina

Pi 0.021

MarkerSeek score 60.00

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 466
variable_sites: 9
pi: 0.021
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	GTGAGGCTCGAAAATGGAGT	TGCTCAAACTCTTCCTTAGACA	50.0	40.9	57.9	57.0	580-599 bp	1.0	60.8
rbcL-accD_p2	GCTGCTTGTGAGGTATGGAA	TGCTCAAACTCTTCCTTAGACA	50.0	40.9	57.9	57.0	548-567 bp	1.0	60.7
rbcL-accD_p3	GAGTCCTGAACTAGCTGCTG	TGCTCAAACTCTTCCTTAGACA	55.0	40.9	57.7	57.0	564-583 bp	1.0	59.3
rbcL-accD_p4	GAGGCTCGAAAATGGAGTCC	TGCTCAAACTCTTCCTTAGACA	55.0	40.9	58.3	57.0	578-597 bp	1.0	58.8
rbcL-accD_p5	GCTCGAAAATGGAGTCCTGA	TGCTCAAACTCTTCCTTAGACA	50.0	40.9	57.6	57.0	575-594 bp	1.0	58.5