Hotspot

psbA-matK

LSC · 1294-1841 · 548 bp

Back to Muellerina

Pi 0.0244

MarkerSeek score 60.00

Primer available yes

Label psbA-matK

Feature Metadata

label_name: psbA-matK
feature_type: igs
region: LSC
length_bp: 548
variable_sites: 13
pi: 0.0244
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA-matK_p1	GGAGCAGCAATGAAGGCTAT	ACAGTTCCAATTCGGCCTTT	50.0	45.0	58.0	58.0	1129-1144 bp	1.0	82.3
psbA-matK_p2	CGCCGTTGTATAACCACTCA	ACAGTTCCAATTCGGCCTTT	50.0	45.0	58.0	58.0	1290-1305 bp	1.0	82.1
psbA-matK_p3	AAGGACCGCCGTTGTATAAC	ACAGTTCCAATTCGGCCTTT	50.0	45.0	58.0	58.0	1296-1311 bp	1.0	82.0
psbA-matK_p4	CTACTGGAGGAGCAGCAATG	ACAGTTCCAATTCGGCCTTT	55.0	45.0	58.0	58.0	1137-1152 bp	1.0	82.0
psbA-matK_p5	CGCCGTTGTATAACCACTCA	AATTCGGCCTTTGGTTGGAT	50.0	45.0	58.0	58.1	1282-1297 bp	1.0	81.7