Hotspot

trnL-UAG-rpl32

SSC · 128419-129174 · 756 bp

Back to Muehlenbeckia

Pi 0.0261

MarkerSeek score 67.80

Primer available yes

Label trnL-UAG-rpl32

Feature Metadata

label_name: trnL-UAG-rpl32
feature_type: igs
region: SSC
length_bp: 756
variable_sites: 35
pi: 0.0261
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 67.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAG-rpl32_p1	TCCTAAGAGCAGCGTGTCTA	TCTCTTTCTACCGGGAATTCT	50.0	42.9	57.9	55.8	431-862 bp	1.0	58.1
trnL-UAG-rpl32_p2	AGCAGCGTGTCTACCAATTT	TCTCTTTCTACCGGGAATTCT	45.0	42.9	57.8	55.8	853-865 bp	1.0	57.1
trnL-UAG-rpl32_p3	CCTAAGAGCAGCGTGTCTAC	TCTCTTTCTACCGGGAATTCT	55.0	42.9	57.8	55.8	859-871 bp	1.0	56.9
trnL-UAG-rpl32_p4	CTAAGAGCAGCGTGTCTACC	TCTCTTTCTACCGGGAATTCT	55.0	42.9	57.8	55.8	858-870 bp	1.0	56.9
trnL-UAG-rpl32_p5	GAGCAGCGTGTCTACCAATT	TCTCTTTCTACCGGGAATTCT	50.0	42.9	58.3	55.8	854-866 bp	1.0	56.3