Hotspot

ndhD-ccsA

SSC · 127022-127231 · 210 bp

Back to Muehlenbeckia

Pi 0.0419
MarkerSeek score 68.90
Primer available yes
Label ndhD-ccsA

Feature Metadata

label_name
ndhD-ccsA
feature_type
igs
region
SSC
length_bp
210
variable_sites
16
pi
0.0419
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.99
markerseek_score
68.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhD-ccsA_p1 CAGTTGAGAAGGTAGAAGCTGT TCATTTCTATTAAAAAGCACCTAA 45.5 25.0 57.7 52.7 281-288 bp 1.0 45.4
ndhD-ccsA_p2 AGTTGAGAAGGTAGAAGCTGT TCATTTCTATTAAAAAGCACCTAA 42.9 25.0 56.3 52.7 280-287 bp 1.0 45.4
ndhD-ccsA_p3 AGTTGAGAAGGTAGAAGCTGTT TCATTTCTATTAAAAAGCACCTAA 40.9 25.0 57.0 52.7 280-287 bp 1.0 45.4
ndhD-ccsA_p4 TGAGAAGGTAGAAGCTGTTCT TCATTTCTATTAAAAAGCACCTAA 42.9 25.0 56.0 52.7 277-284 bp 1.0 45.4
ndhD-ccsA_p5 TCAGTTGAGAAGGTAGAAGCTG TCATTTCTATTAAAAAGCACCTAA 45.5 25.0 57.5 52.7 282-289 bp 1.0 45.3