Hotspot

rpl33-rps18

LSC · 71375-71891 · 517 bp

Back to Muehlenbeckia

Pi 0.0252
MarkerSeek score 67.20
Primer available yes
Label rpl33-rps18

Feature Metadata

label_name
rpl33-rps18
feature_type
igs
region
LSC
length_bp
517
variable_sites
22
pi
0.0252
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.97
markerseek_score
67.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl33-rps18_p1 CTGTCCCTACTGTTCCAAGC GGCCGCTTAGGTTTAAGGAA 55.0 50.0 57.9 57.9 585-607 bp 1.0 85.2
rpl33-rps18_p2 CTGTCCCTACTGTTCCAAGC AAGGCCGCTTAGGTTTAAGG 55.0 50.0 57.9 57.9 587-609 bp 1.0 85.1
rpl33-rps18_p3 CCAGTCGGTTGGAATTGAGA GGCCGCTTAGGTTTAAGGAA 50.0 50.0 57.5 57.9 610-632 bp 1.0 82.1
rpl33-rps18_p4 CCAGTCGGTTGGAATTGAGA AAGGCCGCTTAGGTTTAAGG 50.0 50.0 57.5 57.9 612-634 bp 1.0 82.1
rpl33-rps18_p5 CCCAGTCGGTTGGAATTGAG GGCCGCTTAGGTTTAAGGAA 55.0 50.0 58.5 57.9 611-633 bp 1.0 81.5