Hotspot

accD-psaI

LSC · 61659-62403 · 745 bp

Back to Muehlenbeckia

Pi 0.0211

MarkerSeek score 65.80

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 745
variable_sites: 29
pi: 0.0211
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 65.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	CAGTTGCACGCTTTCTTTCC	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.2	58.0	848-863 bp	1.0	82.5
accD-psaI_p2	CAGTTGCACGCTTTCTTTCC	AAGAAGCCATTGCAATTGCC	50.0	45.0	58.2	57.9	854-869 bp	1.0	81.9
accD-psaI_p3	AGTTGCACGCTTTCTTTCCT	CCATTGCAATTGCCGGAAAT	45.0	45.0	58.3	58.0	847-862 bp	1.0	81.9
accD-psaI_p4	AGTTGCACGCTTTCTTTCCT	AAGAAGCCATTGCAATTGCC	45.0	45.0	58.3	57.9	853-868 bp	1.0	81.3
accD-psaI_p5	TGCACGCTTTCTTTCCTTTG	CCATTGCAATTGCCGGAAAT	45.0	45.0	57.5	58.0	844-859 bp	1.0	80.6