Hotspot

atpB-rbcL

LSC · 57451-58299 · 849 bp

Back to Muehlenbeckia

Pi 0.0245

MarkerSeek score 69.00

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 849
variable_sites: 36
pi: 0.0245
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 69.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	AACCCCAGGACTAGACGTAG	AACACCGGCTTTGAATCCAA	55.0	45.0	57.9	58.3	910-936 bp	1.0	82.3
atpB-rbcL_p2	TGGAAACCCCAGGACTAGAC	AACACCGGCTTTGAATCCAA	55.0	45.0	58.3	58.3	914-940 bp	1.0	80.6
atpB-rbcL_p3	AACCCCAGGACTAGACGTAG	TAACACCGGCTTTGAATCCA	55.0	45.0	57.9	57.4	911-937 bp	1.0	80.1
atpB-rbcL_p4	AACCCCAGGACTAGACGTAG	TGGGGTTCATACTCAGGAGT	55.0	50.0	57.9	57.4	954-980 bp	1.0	79.3
atpB-rbcL_p5	TTTCAAGTGTGGAAACCCCA	AACACCGGCTTTGAATCCAA	45.0	45.0	57.5	58.3	923-949 bp	1.0	79.0