Hotspot

ndhC-trnV-UAC

LSC · 53756-54386 · 631 bp

Back to Muehlenbeckia

Pi 0.0246

MarkerSeek score 63.90

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 631
variable_sites: 28
pi: 0.0246
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 63.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	AGTAGGGGAAACTCAACGGA	TTGGTAGTGCTCCTGGATCA	50.0	50.0	58.0	58.0	1323-1332 bp	0.75	71.4
ndhC-trnV-UAC_p2	TCTCACTAAAGAAGGGGCCA	TTGGTAGTGCTCCTGGATCA	50.0	50.0	58.0	58.0	435-1579 bp	0.75	71.4
ndhC-trnV-UAC_p3	CAATGCTCCTTTTCGCCATG	TTGGTAGTGCTCCTGGATCA	50.0	50.0	58.1	58.0	1226-1235 bp	0.75	71.0
ndhC-trnV-UAC_p4	ACCGAGTTTGGGGTTATTCG	TTGGTAGTGCTCCTGGATCA	50.0	50.0	57.9	58.0	1347-1356 bp	0.75	70.7
ndhC-trnV-UAC_p5	TGGTTCAGGCATTTGCTCAT	TTGGTAGTGCTCCTGGATCA	45.0	50.0	58.1	58.0	1594-1603 bp	0.75	70.6