Hotspot

trnT-UGU-trnL-UAA

LSC · 49804-50299 · 496 bp

Back to Muehlenbeckia

Pi 0.0192
MarkerSeek score 64.00
Primer available yes
Label trnT-UGU-trnL-UAA

Feature Metadata

label_name
trnT-UGU-trnL-UAA
feature_type
igs
region
LSC
length_bp
496
variable_sites
17
pi
0.0192
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-UGU-trnL-UAA_p1 GCGATGCTCTAACCTCTGAG CCGTAGCGTCTACCGATTTC 55.0 55.0 57.9 58.2 557-562 bp 1.0 81.2
trnT-UGU-trnL-UAA_p2 GAGTCGAACCGATGACCATC CCGTAGCGTCTACCGATTTC 55.0 55.0 58.2 58.2 588-593 bp 1.0 80.8
trnT-UGU-trnL-UAA_p3 GCGATGCTCTAACCTCTGAG CGTCTACCGATTTCGCCATA 55.0 50.0 57.9 57.6 551-556 bp 1.0 80.0
trnT-UGU-trnL-UAA_p4 GAGTCGAACCGATGACCATC CGTCTACCGATTTCGCCATA 55.0 50.0 58.2 57.6 582-587 bp 1.0 79.6
trnT-UGU-trnL-UAA_p5 CTAACCTCTGAGCTAAGCGG CCGTAGCGTCTACCGATTTC 55.0 55.0 57.5 58.2 549-554 bp 1.0 78.4