Hotspot

ycf3-trnS-GGA

LSC · 47243-48160 · 918 bp

Back to Muehlenbeckia

Pi 0.0158

MarkerSeek score 66.60

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 918
variable_sites: 27
pi: 0.0158
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	TTCGCCTGAAGTTGTTGGAA	CTGGGTGAAGGTACGGAAAG	45.0	55.0	58.0	57.9	1114-1117 bp	1.0	81.5
ycf3-trnS-GGA_p2	TTTCGCCTGAAGTTGTTGGA	CTGGGTGAAGGTACGGAAAG	45.0	55.0	58.0	57.9	1115-1118 bp	1.0	81.5
ycf3-trnS-GGA_p3	TTGGCCACAACTGAAAAGGT	CTGGGTGAAGGTACGGAAAG	45.0	55.0	58.1	57.9	435-1079 bp	1.0	81.3
ycf3-trnS-GGA_p4	TTCGCCTGAAGTTGTTGGAA	ACGGAAAGAGAGGGATTCGA	45.0	50.0	58.0	58.2	1102-1105 bp	1.0	81.0
ycf3-trnS-GGA_p5	TTTCGCCTGAAGTTGTTGGA	ACGGAAAGAGAGGGATTCGA	45.0	50.0	58.0	58.2	1103-1106 bp	1.0	81.0