Hotspot

trnY-GUA-trnE-UUC

LSC · 32992-33061 · 70 bp

Back to Muehlenbeckia

Pi 0.031

MarkerSeek score 64.30

Primer available yes

Label trnY-GUA-trnE-UUC

Feature Metadata

label_name: trnY-GUA-trnE-UUC
feature_type: igs
region: LSC
length_bp: 70
variable_sites: 4
pi: 0.031
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnY-GUA-trnE-UUC_p1	TGGATTTGAACCAGCGTAGG	CATAGTATGATGGCGGTCGG	50.0	55.0	57.9	58.0	241-246 bp	1.0	82.0
trnY-GUA-trnE-UUC_p2	ATTTGAACCAGCGTAGGCAT	CATAGTATGATGGCGGTCGG	45.0	55.0	57.9	58.0	238-243 bp	1.0	81.9
trnY-GUA-trnE-UUC_p3	TGGATTTGAACCAGCGTAGG	ATAGTATGATGGCGGTCGGA	50.0	50.0	57.9	58.1	240-245 bp	1.0	81.4
trnY-GUA-trnE-UUC_p4	ATTTGAACCAGCGTAGGCAT	ATAGTATGATGGCGGTCGGA	45.0	50.0	57.9	58.1	237-242 bp	1.0	81.3
trnY-GUA-trnE-UUC_p5	AAATGGAATTCTTGGGCCGA	CATAGTATGATGGCGGTCGG	45.0	55.0	57.8	58.0	263-268 bp	1.0	80.8