Hotspot

psbM-trnD-GUC

LSC · 31403-32447 · 1045 bp

Back to Muehlenbeckia

Pi 0.019

MarkerSeek score 65.40

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 1045
variable_sites: 36
pi: 0.019
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	TGTTTTGGCTGACCGTTTTT	TTCAATTGGTCAGAGCACCG	40.0	50.0	57.0	58.5	1191-1216 bp	1.0	71.8
psbM-trnD-GUC_p2	TTGTTTTGGCTGACCGTTTT	TTCAATTGGTCAGAGCACCG	40.0	50.0	57.0	58.5	1192-1217 bp	1.0	71.8
psbM-trnD-GUC_p3	AATTGTTTTGGCTGACCGTT	TTCAATTGGTCAGAGCACCG	40.0	50.0	56.7	58.5	1194-1219 bp	1.0	69.9
psbM-trnD-GUC_p4	ATTGTTTTGGCTGACCGTTT	TTCAATTGGTCAGAGCACCG	40.0	50.0	56.7	58.5	1193-1218 bp	1.0	69.9
psbM-trnD-GUC_p5	TTGTTTTGGCTGACCGTTTTT	TTCAATTGGTCAGAGCACCG	38.1	50.0	57.7	58.5	1192-1217 bp	1.0	69.5