Hotspot

petN-psbM

LSC · 30122-31297 · 1176 bp

Back to Muehlenbeckia

Pi 0.0162
MarkerSeek score 69.90
Primer available yes
Label petN-psbM

Feature Metadata

label_name
petN-psbM
feature_type
igs
region
LSC
length_bp
1176
variable_sites
35
pi
0.0162
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
69.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-psbM_p1 CCCTTTCACTCGTCGTATGG AAAACGGTCAGCCAAAACAA 55.0 40.0 58.1 57.0 1231-1238 bp 1.0 73.9
petN-psbM_p2 CCTTTCACTCGTCGTATGGG AAAAACGGTCAGCCAAAACA 55.0 40.0 58.1 57.0 1231-1238 bp 1.0 73.9
petN-psbM_p3 CCTTTCACTCGTCGTATGGG AAAACGGTCAGCCAAAACAA 55.0 40.0 58.1 57.0 1230-1237 bp 1.0 73.9
petN-psbM_p4 CTTTCACTCGTCGTATGGGG AAAAACGGTCAGCCAAAACA 55.0 40.0 58.1 57.0 1230-1237 bp 1.0 73.9
petN-psbM_p5 CTTTCACTCGTCGTATGGGG AAAACGGTCAGCCAAAACAA 55.0 40.0 58.1 57.0 1229-1236 bp 1.0 73.9