Hotspot

trnS-GCU-trnG-GCC

LSC · 9027-10234 · 1208 bp

Back to Muehlenbeckia

Pi 0.0299

MarkerSeek score 74.20

Primer available yes

Label trnS-GCU-trnG-GCC

Feature Metadata

label_name: trnS-GCU-trnG-GCC
feature_type: igs
region: LSC
length_bp: 1208
variable_sites: 64
pi: 0.0299
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 74.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-GCC_p1	ACGGAAAGAGAGGGATTCGA	ACGAATCACACTTTTACCACT	50.0	38.1	58.2	55.5	527-1331 bp	1.0	59.1
trnS-GCU-trnG-GCC_p2	CGAATGACTCGTACAACGGA	ACGAATCACACTTTTACCACT	50.0	38.1	57.7	55.5	497-1301 bp	1.0	58.4
trnS-GCU-trnG-GCC_p3	ACGGAAAGAGAGGGATTCGA	AGAACGAATCACACTTTTACCAC	50.0	39.1	58.2	57.3	530-1334 bp	1.0	58.0
trnS-GCU-trnG-GCC_p4	ACGGAAAGAGAGGGATTCGA	GAACGAATCACACTTTTACCACT	50.0	39.1	58.2	57.3	529-1333 bp	1.0	58.0
trnS-GCU-trnG-GCC_p5	CGAATGACTCGTACAACGGA	GAACGAATCACACTTTTACCACT	50.0	39.1	57.7	57.3	499-1303 bp	1.0	57.3