Hotspot

rps16-trnQ-UUG

LSC · 6132-7715 · 1584 bp

Back to Muehlenbeckia

Pi 0.0222

MarkerSeek score 66.00

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1584
variable_sites: 63
pi: 0.0222
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 66.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	CGTTGCTTTCTACCACATCG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.2	58.1	1594-1649 bp	1.0	79.8
rps16-trnQ-UUG_p2	GCACGTTGCTTTCTACCACA	GAGGTTCGAATCCTTCCGTC	50.0	55.0	59.1	58.1	1597-1652 bp	1.0	77.6
rps16-trnQ-UUG_p3	CGTTGCTTTCTACCACATCG	TTCGGAGGTTCGAATCCTTC	50.0	50.0	57.2	57.3	1598-1653 bp	1.0	75.1
rps16-trnQ-UUG_p4	CGTTGCTTTCTACCACATCG	TAAGGCAACGGGTTTTGGTC	50.0	50.0	57.2	58.7	1624-1679 bp	1.0	74.9
rps16-trnQ-UUG_p5	CACGTTGCTTTCTACCACATC	GAGGTTCGAATCCTTCCGTC	47.6	55.0	57.5	58.1	1596-1651 bp	1.0	74.5