Hotspot

ycf3

LSC · 42071-44029 · 1959 bp

Pi 0.00104

MarkerSeek score 46.70

Primer available yes

Label ycf3

Feature Metadata

label_name: ycf3
feature_type: gene
region: LSC
length_bp: 1959
variable_sites: 4
pi: 0.00104
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3_p1	TGGCTCTTTCCACAAAACAA	CCACGAGAGGGAATGAGAAG	40.0	55.0	56.0	57.4	2036-2041 bp	1.0	59.4
ycf3_p2	TTGGCTCTTTCCACAAAACA	CCACGAGAGGGAATGAGAAG	40.0	55.0	56.0	57.4	2037-2042 bp	1.0	59.4
ycf3_p3	TGGCTCTTTCCACAAAACAAAG	CCACGAGAGGGAATGAGAAG	40.9	55.0	57.9	57.4	2036-2041 bp	1.0	58.6
ycf3_p4	TCTTTGATTGGCTCTTTCCACA	CCACGAGAGGGAATGAGAAG	40.9	55.0	58.2	57.4	2044-2049 bp	1.0	58.3
ycf3_p5	TGGCTCTTTCCACAAAACAAA	CCACGAGAGGGAATGAGAAG	38.1	55.0	56.8	57.4	2036-2041 bp	1.0	57.6