Hotspot

ndhD

SSC · 114194-115723 · 1530 bp

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Pi 0.00179
MarkerSeek score 40.30
Primer available yes
Label ndhD

Feature Metadata

label_name
ndhD
feature_type
gene
region
SSC
length_bp
1530
variable_sites
6
pi
0.00179
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
40.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhD_p1 TTGTGCAACGAACCCTTTTG TTACCGACAAAATCCGTGCT 45.0 45.0 57.7 57.8 1649-1650 bp 1.0 76.8
ndhD_p2 TCATTGTGCAACGAACCCTT TTACCGACAAAATCCGTGCT 45.0 45.0 58.3 57.8 1652-1653 bp 1.0 76.5
ndhD_p3 TTGTGCAACGAACCCTTTTG TACCGACAAAATCCGTGCTC 45.0 50.0 57.7 58.3 1648-1649 bp 1.0 75.9
ndhD_p4 TCATTGTGCAACGAACCCTT TACCGACAAAATCCGTGCTC 45.0 50.0 58.3 58.3 1651-1652 bp 1.0 75.7
ndhD_p5 TTGTCATTGTGCAACGAACC TTACCGACAAAATCCGTGCT 45.0 45.0 57.5 57.8 1655-1656 bp 1.0 75.2