Hotspot

ndhA

SSC · 118317-120449 · 2133 bp

Pi 0.00285

MarkerSeek score 37.50

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2133
variable_sites: 15
pi: 0.00285
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 37.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	CCCTGACCAATGTATCTGGC	CAATGTTACGAGCTTCCGGA	55.0	50.0	58.0	58.0	2806-2809 bp	1.0	80.3
ndhA_p2	TGACCAATGTATCTGGCAGC	CAATGTTACGAGCTTCCGGA	50.0	50.0	58.0	58.0	2803-2806 bp	1.0	80.2
ndhA_p3	CCCTGACCAATGTATCTGGC	TATTGGCGGAGAAGAAGCAC	55.0	50.0	58.0	58.0	2847-2850 bp	1.0	80.2
ndhA_p4	CAATGTATCTGGCAGCTCGT	CAATGTTACGAGCTTCCGGA	50.0	50.0	58.1	58.0	2799-2802 bp	1.0	80.1
ndhA_p5	TGACCAATGTATCTGGCAGC	TATTGGCGGAGAAGAAGCAC	50.0	50.0	58.0	58.0	2844-2847 bp	1.0	80.0