Hotspot

rpl20-rps12

LSC · 68826-69636 · 811 bp

Back to Mosla

Pi 0.00119
MarkerSeek score 47.00
Primer available yes
Label rpl20-rps12

Feature Metadata

label_name
rpl20-rps12
feature_type
igs
region
LSC
length_bp
811
variable_sites
2
pi
0.00119
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
47.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl20-rps12_p1 ACCCTCGAAAACTTGATGCA CGCCGAGGAACATGTACTAG 45.0 55.0 57.7 57.9 927-931 bp 1.0 77.4
rpl20-rps12_p2 CGGGCTATATATCCGCGTTT CGCCGAGGAACATGTACTAG 50.0 55.0 57.7 57.9 880-884 bp 1.0 77.3
rpl20-rps12_p3 ACCCTCGAAAACTTGATGCA ACTAGGGTGTATGTGCGACT 45.0 50.0 57.7 58.2 912-916 bp 1.0 77.2
rpl20-rps12_p4 CGGGCTATATATCCGCGTTT ACTAGGGTGTATGTGCGACT 50.0 50.0 57.7 58.2 865-869 bp 1.0 77.0
rpl20-rps12_p5 ACCCTCGAAAACTTGATGCA AATACAAGACAGCCCGTTCG 45.0 50.0 57.7 58.3 987-991 bp 1.0 76.1