Hotspot

petA-psbJ

LSC · 63127-64178 · 1052 bp

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Pi 0.00426
MarkerSeek score 53.40
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
1052
variable_sites
13
pi
0.00426
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
53.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 GGCGTCTGTTATTTTGGCAC TCCGGATTAGGTTCATCCCT 50.0 50.0 58.0 57.5 1155-1166 bp 1.0 78.3
petA-psbJ_p2 GGCGTCTGTTATTTTGGCAC CCGGATTAGGTTCATCCCTG 50.0 55.0 58.0 57.4 1154-1165 bp 1.0 77.8
petA-psbJ_p3 GAGAAGGTTCAATTGGCCGA TCCGGATTAGGTTCATCCCT 50.0 50.0 58.2 57.5 1103-1114 bp 1.0 77.2
petA-psbJ_p4 TCGAGAAGGTTCAATTGGCC TCCGGATTAGGTTCATCCCT 50.0 50.0 58.2 57.5 1105-1116 bp 1.0 77.2
petA-psbJ_p5 GAGAAGGTTCAATTGGCCGA CCGGATTAGGTTCATCCCTG 50.0 55.0 58.2 57.4 1102-1113 bp 1.0 76.7