Hotspot

trnT-GGU-psbD

LSC · 31151-32461 · 1311 bp

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Pi 0.00132
MarkerSeek score 47.20
Primer available yes
Label trnT-GGU-psbD

Feature Metadata

label_name
trnT-GGU-psbD
feature_type
igs
region
LSC
length_bp
1311
variable_sites
4
pi
0.00132
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
47.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-psbD_p1 GTGGTAGAGTAACGCCATGG ACAAAAACGAAACGGTCCCT 55.0 45.0 58.1 58.2 1455-1461 bp 1.0 78.1
trnT-GGU-psbD_p2 GTGGTAGAGTAACGCCATGG CCCTCCGTAACCAATCATCC 55.0 55.0 58.1 57.7 1439-1445 bp 1.0 78.0
trnT-GGU-psbD_p3 TCAGTGGTAGAGTAACGCCA ACAAAAACGAAACGGTCCCT 50.0 45.0 58.1 58.2 1458-1464 bp 1.0 77.9
trnT-GGU-psbD_p4 TCAGTGGTAGAGTAACGCCA CCCTCCGTAACCAATCATCC 50.0 55.0 58.1 57.7 1442-1448 bp 1.0 77.9
trnT-GGU-psbD_p5 GTGGTAGAGTAACGCCATGG GCCAGACCAGCCTACAAAAA 55.0 50.0 58.1 58.4 1468-1474 bp 1.0 77.0