Hotspot

petN-psbM

LSC · 28433-29463 · 1031 bp

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Pi 0.00285
MarkerSeek score 52.00
Primer available yes
Label petN-psbM

Feature Metadata

label_name
petN-psbM
feature_type
igs
region
LSC
length_bp
1031
variable_sites
7
pi
0.00285
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
52.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-psbM_p1 ACTCGTAGTGTGGGGAAGAA TCCGACCGCCTTTCTACTTA 50.0 50.0 58.0 58.2 1113-1117 bp 1.0 79.5
petN-psbM_p2 TCGTAGTGTGGGGAAGAAGT TCCGACCGCCTTTCTACTTA 50.0 50.0 58.0 58.2 1111-1115 bp 1.0 79.5
petN-psbM_p3 ACTCGTAGTGTGGGGAAGAA TAGTTCCGACCGCCTTTCTA 50.0 50.0 58.0 58.2 1117-1121 bp 1.0 79.4
petN-psbM_p4 TCGTAGTGTGGGGAAGAAGT TAGTTCCGACCGCCTTTCTA 50.0 50.0 58.0 58.2 1115-1119 bp 1.0 79.4
petN-psbM_p5 CGTAGTGTGGGGAAGAAGTG TCCGACCGCCTTTCTACTTA 55.0 50.0 57.9 58.2 1110-1114 bp 1.0 78.9